Porcine peroxisome proliferator-activated receptor γ induces transdifferentiation of myocytes into adipocytes1
نویسندگان
چکیده
Peroxisome proliferator-activated receptor γ2 (PPARγ) is a nuclear transcription factor that regulates adipocyte differentiation and lipogenic genes during adipogenesis. The activity of rodent PPARγ is regulated by phosphorylation of serine 112. The current experiment was designed to study the ability of porcine PPARγ to stimulate transdifferentiation of myoblasts to adipocytes by overexpressing wild-type PPARγ or mutated PPARγ (serine 112 was mutated to alanine) in mouse myoblast cells. The expression of adipogenic marker genes (adipocyte fatty acid binding protein, lipoprotein lipase, and glycerol-3 phosphate dehydrogenase) in cells stably expressing mutated porcine PPARγ was greater than in cells with wild-type PPARγ, indicating that the mutated PPARγ has greater adipogenic
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